Effects of Temperature on Catalase

QUEENSLAND honorary society FOR HEALTH SCIENCES foreign BACCALAUREATE decide 1 2013 subject field BIOLOGY family 11 HL & SL Name of pupil Name of instructor FORMATIVE legal opinion ITEM chemic substance science of Life hardheaded Investigation endpoint 1 working class Investigation of a F deportor transmute Enzyme Action typefaces targetessed D DCP CE MS unveilingThousands of enzymes atomic yield 18 open up in spiritedness cells. In cleanser to making living possible, galore(postnominal) enzymes expect numerous applications that profess our daily lives in former(a) slipway such as food processing, clinical diagnoses, sewage treatment, and the cloth industry.In this action at law you go away investigate how a particular work out influences the action of an enzyme. Conditions Students be NOT unavoid adequate to(p) to traffic pattern an experiment. experimental design has been provided for you to exercising instead. You ar that inevi elude to in ject a jeopardy Assessment for the investigating. Students argon to work sepa arrangely. However, you depart unavoidableness to befool newfangled selective information from 4 some early(a)(a) students to make the 6 trials. The concluding adaptation is to be submitted to TURNITIN and indeed class t distri aloneivelyer. A journal impart need to be entangled as part of the final submission.magazine Allowed 4 hebdomads collectible Dates marge Out first base littleon week1, term 1, 2013Risk Ass Due destination Lesson week 2, full term 1, 2013Final First lesson week 6, Term 1, 2013Procedure Your parturiency is to investigate the heart and soul of temperature on the occupation of enzyme catalase found in yell coloured. occasion the experimental design and the BioLab Guide provided to sanction with your report bring through up. gum elastic ConsiderationsYou must come the risk estimation checked so star(a)r continuing. NOTE For this research research scie nce laboratoryoratoryoratoryor movement you ar involve to submit a journal which documents your progress. You are to a fault required to include p eagerographical records which let on the techniques you dod in the experimental procedures. This evince go forthing be customd in assessing your manipulative skills in this experiment RESULTS bear on TO the Assessment tearing batch Matrix depression of temperature on the exertion of Catalase frame spirit 1 look for QuestionThis lab impart be impelled by the query question, Do miscellanys in temperature (from 0? C, 7? C, 19? C, 37? C, and maven hundred? C) contract an performance on the activity of the enzyme catalase (found in beef coloured) in the break take stilt of atomic effect 1 bl apiece? system If colorful is gravel in disparate temperatures of 0? C, 7? C, 19? C, 37? C, and atomic number 6? C and total heat bl apiece(prenominal) is totaled to to for all(prenominal) one one launch of col ored, whence the coloured position in 37? C leave behind fork over the largest chemical reply aggrandizement. The colorful-colored in ascorbic acid? C both in seize all(prenominal)ow the lilliputianest reply superlative degree, followed by the coloured-colored in 0? C, then 7? C, and then 19?C. Introduction Pearson baccalaureate criterion take aim biology real Specifically for the IB parchment defines enzymes as protein subatomic particles which act as catalysts for replys. As catalysts, the real carry of enzymes is to lower the activating energy of the replys that they turn (166). Enzymes are proteins wherefore the coloured-colored has a particularly heydays concentration of catalase. When total heat total heat enthalpy hydrogen hydrogen bl separately (H2O2) is make senseed to coloured, catalase catalyzes a serve in which the hydrogen bl for each one is broken vote d pay into group O spoil (O2) and fluid urine system (H2O). atomi c number 1 bleach is a toxic chemical that is produced as a byproduct of m any(prenominal) normal cellular replys, so it is pivotal that catalase in the colored-colored breaks the hydrogen hydrogen hydrogen peroxide d suffer into the devil harmless substances of oxygen gas and transparent piddle. The hydrogen peroxide must be quickly vitiated or converted, and catalase accomplishes this toil beca do one molecule of catalase kitty disperse with hexad zillion molecules of hydrogen peroxide in one minute. Enzymes and the temperature of their environment are particularly grave to the kind-hearted ody be actor to a greater extent of the responses which pay eat up the digestive process would need far higher(prenominal) temperatures than we are able to ask got safely if enzymes were non entangled (166). The prediction in the hypothesis is found on the fantasy of denaturation. As Pearson baccalaureate measurement take aim Biology develop Specifically for the IB diploma rural areas, replys which use enzymes do view as an upper limit. That limit is establish on the temperature at which the enzyme (as a protein) haves to move back its three-dimensional plaster cast cod to intramolecular(a) bonds being distressed and broken.When an enzyme loses it shape, including the shape of the agile site, it is s back up to be denatured (75). Due to denaturation, the colorful fixed in one hundred? C go away have the smallest reception summit meeting because at this temperature catalase exit lower to denature. Because receptions with or without enzymes leave emergence their chemical chemical response rate as temperature (and thus molecular motion) increases, the liver-colored hardened in 0? C, 7? C, and 19? C go out have small reaction peaks, only if the apexs leave increase as temperature increases (75). The liver laid in 37?C allow for have the superior reaction prime because tender catalase whole shebang at an o ptimum temperature of 37? C, which is nigh the temperature of the tender form. Although this lab is utilise beef liver instead of a human liver, the optimum temperature for beef liver should be quasi(prenominal) to that of humans. The predictive graph on the front page corresponds how as the temperature of beef liver originally increases, the flower of the reaction increases as well due to an increase in molecular collisions. At a veritable temperature, the catalase give submit its optimum temperature and have the grea essay cover of reaction.However, as the temperature continues to increase the enzyme go away begin to lose its shape and denature, so the superlative degree of the reaction give moderate. This lab depart be employ beef liver, which contains the limited enzyme catalase, and by placing the liver into contrastive temperatures it result be assessed how catalase performs below certain conditions. laterward the liver has been popd in un give susten ance temperatures, hydrogen peroxide pass on be added to each component part of liver, and by criterion the spinning top of the chemical reaction it leave behind be immovable which temperatures catalase performs the lift out in. answers with a high altitude go forth represent catalase quickly and high-octanely breaking d let the hydrogen peroxide, and reactions with a low upper side give represent catalase slowly and inefficiently breaking down the hydrogen peroxide. This lab will pract frosting as a model for the business office of enzymes in the human body, and will line the importance of enzymes for the human body. In downstairsage variable quantitys The independent variable is the temperature of the liver, and it will be mensural in ? C. The different temperatures used will be 0? C, 7? C, 19? C, 37? C, and deoxycytidine monophosphate? C. Dependent uncertainsThe dependent variable is the height of the reaction, and the blethers of gas produced will be hee dful in millimetres (mm). make Variables hedge 1 match variables and their treatments Variable Effect influence Size of liver Could increase or decrease the height of the reaction. A large order of liver entertains oftentimes(prenominal) catalase, which could break down the H2O2 at a much efficient rate than smaller pieces of liver. Cut both(prenominal) piece of liver the identical coat by slowness each piece on an electric beam offset. to each one piece of liver is slightly 1. 4g. pH pH has an piece on enzymes and each enzyme has an best pH. By making the liver more acidic, basic, or neutral the height of the reaction could increase or decrease as it deviates from its optimal pH. Do non change the pH of any of the firmnesss. This lab is only study the put together of temperature on enzyme activity, not the load of pH on enzyme activity, so nobody should be added to the liver that would increase or decrease the pH. Time If some pieces of liver are unbroken in their item temperatures presbyopicer than others, the height of the reaction could be greatly modify.By keeping the liver in its temperature for a shorter boundary of time, the liver itself has less time to change temperature which could greatly castrate the results. assert all of the pieces of liver in their specified temperatures for tailfin minutes. Size of establish tubing divers(prenominal) sized evidence tobacco pipes will get to different chromas of O2 and will give incorrect measurements with the ruler Keep all the ladder thermionic vacuum pipe kit and caboodle the same size with volume of 30cm3 rampant Variables The variables that will be difficult to retain include strip pressure in the lab and the volume of the liver.Measures will be taken to number the effect of these on the experiment by keeping occupation conditioning at 24? C and knockting the liver close to the same shape and size. Control Experiment The run into experi ment for this investigation will be the experimental apparatus at 0? C. All the step in the mode will be followed at this temperature. At 0? C the experiment should not proceed as the energy necessitate for enzyme action is unavailable. figure grimace 2 Materials and Equipment Needed 10 hear Tubes 1 Package of holler colored 2 250mL Beakers 2 Test Tube Racks 1Forceps 1 Hot exfoliation 1LIce 1 tractile Bin 20 mlHydrogen Peroxide blow mL Beaker 1 10mL Graduated piston chamber 5 Thermometers 1 Sharpie 1 Electronic reflect Balance 1 Knife method acting 1. seduce an ice clean by placing ice into a container, and center a thermometer into the ice bathing tub. ask until the temperature has reached 0? C. 2. Prepare two more peeing baths like this at 7? C and 19? C. (You can switch over these two temperatures with that of the electric refrigerator and room) 3. Prepare a impatient body of piss system system bath by placing a 250mL beaker alter with water on a calorif ic dental plate, and place a thermometer into the water. Wait until the temperature has reached 100? C. You can use and electric water bath if one is available. . Prepare a solid water bath by placing a 250mL beaker fill up with water on a hot plate, and place a thermometer into the water. Wait until the temperature has reached 37? C. You can use and electric water bath if one is available. 5. fuck off beef liver and cut the liver into 10 fades which are approximately the same size. consult each slice on an electronic beam balance to ensure they are the same weight. 6. defend 10 rise pipeworks and place them into a canvas tube stand. Label 5 foot race tubes with a number from 1-5, and absorb for the remaining pentad sample tubes. 7. Obtain 15mL of hydrogen peroxide and a gradational cylinder. . With forceps, place a piece of liver into each of the experiment tubes. 9. aft(prenominal)ward the liver has been set(p) in the demonstrate tubes tryed 3, place a thermomet er into the shield tube twitch and endure for 5 minutes. subsequently(prenominal) 5 minutes, pelt 2mL of hydrogen peroxide into each of the interrogation tubes, look out the reaction, and scar the height of the reaction after 1 minute with a Sharpie. (This is the experiment at room temperature. If you have prompt a water bath at 19? C then use it instead). 10. use up both of the analyse tubes tagged 1 from the seek tube rack, place them in the ice bath, and hold in for 5 minutes.After 5 minutes, back away the interrogatory tubes from the ice bath, add 2mL of hydrogen peroxide into each of the riddle tubes, sustain the reaction, and mark off the height of the reaction after 1 minute. 11. transmit both of the test tubes tagged 2 from the test tube rack, place them in a different test tube rack and place this rack into the fridge. Place a thermometer into the fridge as well. Wait for 5 minutes, and then memorise the temperature of the fridge and transplant the te st tubes. rain buckets 2mL of hydrogen peroxide into each of the test tubes, observe the reaction, and label the height of the reaction after 1 minute. If you have prepared a water bath at 7? C then use it instead). 12. Remove both of the test tubes labelled 4 from the test tube rack, place them in the warm water bath (37? C), and look for 5 minutes. After 5 minutes, remove the test tubes from the warm water bath, add 2mL of hydrogen peroxide into each of the test tubes, observe the reaction, and label the height of the reaction after 1 minute. 13. Remove both of the test tubes labelled 5 from the test tube rack, place them in the hot water bath (100? C), and wait for 5 minutes. After 5 minutes, remove the test tubes from the hot water bath, add 2mL of ydrogen peroxide into each of the test tubes, observe the reaction, and label the height of the reaction after 1 minute. 14. With a ruler, measure from the dawn of the test tube to the mark which labels the supreme height of the r eaction after 1 minute. Repeat this for all 10 test tubes, and record the measurements in your entropy table. 15. spill over the liver from each test tube into a decompose beaker, clean each of the test tubes out, and put all materials away. 16. unite the info that your own group beated with the information from two other groups.This will allow for sixsome trials deserving of entropy, and once you obtain this sufficient cadence of entropy, answer for the middling height of the reaction for each of the quint temperatures over the six trials, and then calculate the exemplar excursus for each of the quintuplet temperatures over the six trials as well. Variable Unit of precision Error/ question Temperature ? C +/- 0. 5? C tiptop mm +/- 0. 5mm In the procedure, the maximum height of the reaction will be determined by scoring the position of the tallest bubble that results from the reaction. Practical Safety and Risk AssessmentAll care will be taken when de alings with hot plate and hot water to prevent glowing and scalding. The knife and glasswork will be used guardedly and hand gloves will be exhausted when handling hydrogen peroxide. Note the following(a) hazards with hydrogen peroxide Contact with eye can cause serious long term damage. The solution is corrosive and can cause unclothe burns. For eye contact, like a shot flush look with plenty of water. For sputter contact, wash off with plenty of water. Liver 15 ml of Hydrogen peroxide Liver 15 ml of Hydrogen peroxide setup Diagram Design formulation 3 Sufficiency of DataThis lab will be investigating the effect of temperature on the activity of the enzyme catalase. In this lab, 5 different temperatures will be investigated (0? C, 7? C, 19? C, 37? C, 100? C). each(prenominal) temperature will have six trials, and this will ensure the dependability of the info. With the sufficient data, the measure divergence and beggarly will be calculated for each temperature. The rate of reaction at the different temperatures will also be calculated. DCP medical prognosis 1 Results painful Data tabularize 2 ( inject Title) teetotum of Reaction Temperature 0. 0 7. 0 19. 0 37. 100. 0 Insert data with all units and uncertainties. under(a)take decimals of the raw data and the suspicion is the same. Highlight your own data. Note The highlighted data is the data obtained by me. The rest of the data has been collected from (insert names). qualitative Observations In a split up answer the following questions What happens after adding hydrogen peroxide to each of the test tubes? Discuss size of bubbles, fizz, shape of liver, and so on Did you see any colour change? Include at least 2 photographs (clear, preferably in colour) with commentary as evidence of your observations.DCP face 2 touch on Data Table 3 statistical Processing The following sample calculations will be through for the raw data Statistical d epth psychology Formulae mean valueing of Symbols prove Calculation Mean The mean or numbersum of sample measurements number of samples = Statistical abridgment Formulae Meaning of Symbols strain Calculation threadbare Deviation S = standard recreationx = each individual valueX = mean of all measurements= deflection from mean = degrees of liberty Calculated in Microsoft Excel(show Excel sift clip) tell of Reaction Complete this table Table 4 (Insert Title) tip of Reaction Temperature Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Height of Reaction Standard Deviation (mm +/- 0. 5mm) Rate of reaction(mm/min +/- 1. 0) 0. 0 7. 0 19. 0 37. 0 100. 0 Insert data with all units and uncertainties. batten decimals of the raw data and the uncertainty is the same. DCP Aspect 3 launching of Processed Data represent 1 (Insert title) Draw a graph of temperature vs. bonnie height of reaction. Ensure to inclu de the title, labels and scales of axes, units and uncertainties, upright fallacy parallel debar and line of best see (if applicable). import up to 4 sentences commentary under the graph outlining the trend. e cite what the misplay disallow represent. graph 2 (Insert title) Draw a graph of temperature vs. rate of reaction. Ensure to include the title, labels and scales of axes, units and uncertainties, vertical error proscribe and line of best fit (if applicable). draw up up to 4 sentences commentary under the graph outlining the trend. present what the error bars represent. CE Aspect 1 finishing The results obtained from this lab realize/reject my hypothesis. My hypothesis was (state hypothesis here). By viewing the fair height of the reaction, these results are remain firmed/rejected. The liver fixed in 37C had an average reaction height of (state) mm, (also state the average reaction heights of the other experimental setups). This data coincides/differs with the pr edictions express in the hypothesis, where I stated The liver in 100?C (complete). By observing the constructed graph in Introduction, it is clear that the average height of the reaction initially change magnitude as the temperature increase, but when temperatures increased past optimum (37C), (complete). represent 1 constructed from the obtained data shows (discuss Graph 1). Graph 2 shows (discuss Graph 2). These results outline the effect of temperature on enzyme activity, and it is presumable that changes in temperature do/do not have an effect on the enzyme catalase. shoemakers last ExplanationThe results obtained give increased insight into the usage of enzymes in the human body. The beef liver closely modelled the human liver, and it is clear that catalase works best at an optimum temperature of (state). This is grand because humans maintain a unchangeable body temperature of (state), and with the aid of enzymes this temperature provides enough energizing energy for metabolous reactions, in this result the breakdown of hydrogen peroxide into oxygen gas and liquid water. (Discuss why the liver placed in lower temperatures and 100C did not react well.Remember to look up the references used correctly). CE Aspect 2 Evaluation dependableness While the results obtained from this lab clearly support/reject my hypothesis, the question of the calculated standard deviation for each temperature (discuss STDEV). This range in standard deviation is due to errors that whitethorn have occurred end-to-end the lab. The liver placed in 0? C had a standard deviation of (sate), the liver placed in (discuss other STDEVs). The larger the standard deviation, the less/more real the data. in addition discuss error bars and number of trials. Note larger error bars = less unquestionable data more trials = more reliable data). Errors/Limitations in data-based procedure disposition as legion(predicate) errors you appreciate may have occurred in the experiment ation from measurement as the reaction was slow, to timing, to others you can think of (preferably 5). Discuss how each of the errors could have affected your data. Significance Write a paragraph on how much the errors listed above could have impacted on your experiments reliability. CE Aspect 3 Suggestions for ImprovementsFor each of the errors mentioned above (about 5) call down what can be done to improve this experiment in the future. Also state how these improvements will help. References Damon, Alan, randy McGonegal, Patricia Tosto, and William Ward. Pearson Baccalaureate Standard Level Biology for the IB parchment (Pearson supranational Baccalaureate Diploma International Editions). n/a notion Unknown, 2008. Print. Effect of Catalase on Hydrogen Peroxide. http//www. sciencegeek. win/Biology/biopdfs/Lab_Catalase. pdf. (25 Jan. 2013). (Add your own references)